Generator

Part:BBa_I766258

Designed by: Nili Sommovilla   Group: iGEM07_UCSF   (2007-10-25)

OspF-EE under inducible promoter

OspF-EE under inducible promoter

Usage and Biology

OspF is a bacterial phosphothreonine lyase, apparently specific for MAPKs. It catalyzes the removal of the phosphorylated threonine side chain present in the MAPK activation loop, therefore inactivating the kinase in an irreversible manner. Here, OspF is N-terminally fused to mCherry, and C-terminally to the synthetic leucine zipper EE; being expressed under control of a Fig1 promoter (inducible by mating pathway activation).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1540
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1540
    Illegal NotI site found at 2099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1540
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1540
    Illegal NgoMIV site found at 172
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None